🧫 Culturing microorganisms

Growing microorganisms has been a fundamental element of much of experimental biology, as well as the underpinning of many modern molecular biology techniques. Perhaps we have a sample of earth that we want to analyse to find a new microorganism with antibacterial properties. Perhaps we are testing a patient sample for an infectious agent. Most likely, we are culturing a safe strain of E. coli that has been genetically modified to produce a protein of interest like human insulin that we can isolate from it and administer to patients.


Aseptic technique

Aseptic means free of contamination. There are hundreds of fungal spores in the air we breathe at all times. There are bacteria and viruses everywhere. If we are to culture Escherichia coli (bacteria, prokaryote) or perhaps Pichia pastoris (yeast, eukaryote), we’re going to be feeding them some nice nutrients, and chances are, loads of other microorganisms will jump at the opportunity to feast.


We don’t want contamination, we just want our specific species that we are culturing and nothing else. The various techniques employed to this end have evolved through time and can even differ between labs and scientists:


A flame (Bunsen burner) can be used in the close vicinity of handling the target microorganism and related equipment and reagents, in order to make the surrounding air warm up and rise higher, carrying away…