All UK Exam Boards included

Mass transport in plants

Download Topic


Water enters a plant through the roots. In order to understand how water gets in the root, you should definitely check out the root structure:



What you can see above is a delicious slice of pineapple. OK, it’s not. That is a slice of a root. Roots, as you may have seen in real life, are hairy. All those tiny and not so tiny root hairs buried into the soil greatly increase the surface area of the root. This exposes it to more water molecules which can be taken up. The hairs are nothing like human hairs; they are extensions of the outer layer of the root, made up of cells. This layer is called the epidermis.

Why does water move inside the root? Simple: osmosis. The cell sap (i.e. cell juice) has a lower water potential than the fluid found in the soil, so the water in the soil kindly makes its way into the thirsty awaiting root. Once the water reaches the first cell in its path, the water potential of that cell is increased compared to the cell next to it. Therefore, water moves into the next cell, leaving the current cell. This in turn results in the previous cell taking up water all over again, and so forth, until water makes its way across all cells of the cortex.


Reaching the endodermis, water then enters the xylem. The xylem is a tissue of dead cells which contributes to the vascular system of plants by being the transportation medium for water and dissolved mineral ions. The xylem brings them to the leaves and plants’ other organs.

There are two different pathways that water uses in order to reach the xylem:


1. The apoplast pathway whereby water slaloms between cell walls and the spaces in between, without passing directly through live tissue; this accounts for 90% of water uptake.

2. The symplast pathway whereby water goes straight through living tissue i.e. the cells in the cortex, and into the xylem; this accounts for only 10% of water uptake.


Basically, the symplast pathway is just way simpler.




Transpiration is water loss through the parts of a plant which are found above soil level i.e. not the roots. As water streams through a plant, transpiration affects the speed of the stream. Increased transpiration will lead to a quicker uptake of water through the roots to maintain the water flow throughout the plant. So what affects transpiration?


1. Light causes stomata to open, resulting in increased water loss (transpiration).

2. Temperature going up also raises the rate of transpiration, as more water molecules evaporate.

3. Humidity. An increase in humidity around the leaves means that there is less space for water molecules from the plant to evaporate into, so transpiration is decreased.

4. Air movement (wind) can displace water molecules from around the stomata, so that more space becomes available for additional water molecules to go into. Transpiration increases.


Root pressure. The cohesion-tension hypothesis


These are the two ways in which the stream of water through a plant can work. Root pressure is the water being pushed into the roots, while cohesion-tension is the water being pulled up.


When a plant doesn’t transpire much, mineral ions can get accumulated at the bottom in the roots. This decreases the water potential inside the roots, so that water moves in by osmosis from the soil into the roots.


A key property of water is cohesion. Cohesion refers to the way in which water molecules stick to one another. A good example of this is when water moves up a very narrow plastic tube, all by itself. This is due to water sticking to itself and hence pulling itself upwards. This happens in plants too.


Investigating transport in plants


There are a couple of neat experiments that can help figure out how things move around in a plant. One involves getting the leaves to use tracers like radioactive carbon (C14 isotope) in carbon dioxide for their photosynthesis.


You could set this up in a bag for example sealed around a leaf to make sure it doesn’t diffuse too far away from the leaf, and then use X-rays to visualise where in the plant the C14 ends up (source to sink routes such as leaf to root). The carbon dioxide containing the C14 will be used to make carbohydrates which then end up elsewhere in the plant.


Another method of investigating plant transport called ringing involves cutting the outermost layer of a plant stem where the phloem is located and then observing changes over time. An accumulation of sugar will be noticeable above the cut ring area, indicating that the movement of molecules would have taken place downwards. Cutting away the phloem disables this, so it simply accumulates at the top of the ring.



Ok byeeeeeeeeeeeee





Sorry! There are no posts.

Sorry! There are no posts.

Your Reviews

Hi! I have recently finished my first year doing biology at uni and I subscribed to you back when I was doing AQA A levels (glad I don’t live in Wales after watching this vid lol) and you and your website helped me so much. Just wanted to say it’s so nice to see you

Mr Peanutbutter YouTube

OMG that’s great! Actually just helped me with my homework

mabelbarc The Student Room

Just a huge thank you for spending your time helping others. I love your site and I'm seriously very grateful. No word of a lie

Neuron13 The Student Room

Thank you for the help, your website and videos are awesome

pika mart YouTube

Thank you!! Your site is so helpful!

studyaspect YouTube